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Image Search Results
Journal: Scientific Reports
Article Title: A novelty-retrieval-extinction paradigm leads to persistent attenuation of remote fear memories
doi: 10.1038/s41598-020-60176-2
Figure Lengend Snippet: Exposure to an OF before the retrieval-extinction session increased hippocampal H3K9/14 acetylation. ( A ) Representative images showing immunostaining for H3K9/14ac in the hippocampal CA3 region 1 h after completion of extinction in nov-Ret and control mice. Higher magnification images of H3K9/14ac staining in the CA3 are shown for the control (b) and nov-Ret groups (d) and correspond to the labelled regions in a and c, respectively. Scale bar: 100 μm. ( B ) Immunohistochemical analysis of OD for H3K9/14ac in the hippocampus for each group. n = 4 mice in each group. ( C , E ) Pictures of western blotting analysis of H3K9/14ac in the hippocampus 1 h after completion of extinction in control and nov-Ret mice. control, n = 5; Ret, n = 2; nov, n = 2; nov-Ret, n = 6. Two mice in the nov-Ret group received fear conditioning and retrieval-extinction in the same context (AA), and another four in different contexts (AB). Original western blotting images are shown in Supplementary Fig. . ( D , F ) Densitometric analysis for H3K9/14ac in ( C , E ), respectively. Data are presented as mean ± SEM. ** P < 0.01, *** P < 0.001 vs . control. OF, open field; SEM, standard error of mean.
Article Snippet: The membranes were blocked with 5% non-fat milk for 2 h at room temperature and incubated overnight with the following antibodies: rabbit polyclonal anti-histone H3 antibody (Servicebio; dilution, 1:30000) and
Techniques: Immunostaining, Staining, Immunohistochemical staining, Western Blot